How To Use Imagej Western Blot
A quick and dirty tutorial on how to quantify a gel image using imagej. Select the widest band in the image as the first band by using rectangular select tool.
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Don't forget to bookmark how to use imagej western blot using Ctrl + D (PC) or Command + D (macos). If you are using mobile phone, you could also use menu drawer from browser. Whether it's Windows, Mac, iOs or Android, you will be able to download the images using download button.
Lukemillerorg Blog Archive Analyzing Gels And Western
1 scanning the western blot film it is best to use a transparency scanner scanner with light source on both sides of the bed.
How to use imagej western blot. Selected areas height should be twic. The area selected should be about twice as tall as it is wide and should be as wide as the widest band on the blot. Well use imagejs gel analysis routine to quantify the density and size of the blots and use the results from our loading controls lower bands to scale the values for our protein of interest upper bands.
Open the western blot image in imagej. I am also looking for alternative techniques to quantify protein bands. Make sure that the image is in 8 bit mode.
Open your image file 2. From the file menu open the image file for the pcna blot you obtained from the chemidoc system. With this kind of scanner when the minimum image area is selected the scanner minimizes the.
Mrkick ass 197199 views. I have quite a number of western blots to be quantified. Open imagej from the desktop or program menu.
How can i properly quantify western blots using imagej. Select a rectangular area around the first band using the rectangular select tool. Western blot analysis with imagej part 1 duration.
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