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Imagej Tutorial For Western Blot
Tutorials by microscope vendors. If youre looking for a more comprehensive workflow option for your western blot analyses please visit my tutorial on using image studio lite a free software.
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Densitometric Analysis Using Nih Image
A quick and dirty tutorial on how to quantify a gel image using imagej.
Imagej tutorial for western blot. The quantification will reflect the relative amounts as a ratio of each protein band relative to the. Selected areas height should be twice of the widest band on the image. Mrkick ass 197199 views.
The following information is an updated version of a method for using imagej to analyze western blots from a now deprecated older page. This video is a quick and dirty tutorial on how to quantify a gel image using nihs imagej a free image analysis software. Karios instruments has a number of interesting fiji tutorials the same page shows tutorials on using cellprofiler too.
Open your image file 2. A quick and dirty tutorial on how to quantify a gel image using. Imagej videos and fiji videos on youtube.
This is very simple method to calculate your protein ratio from western blot images using imagej and excel. Tutorials for software developers and scripters. Western blot analysis with imagej part 1 duration.
Quantifications of western blots with imagej by hossein davarinejad this protocol will allow you to relatively no absolute values quantify protein bands from western blot films. Select the widest band in the image as the first band by using rectangular select tool.
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